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. 2021 Oct 4;2:754501. doi: 10.3389/falgy.2021.754501

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Copyright © 2021 Gehring, Wieczorek, Kapp and Wedi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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TCNs overcame the survival prolonging effects of SEA, SEB, SEC, and of key pro-survival eosinophil cytokines, i.e., a mixture of IL-3, IL-5, and GM-CSF (ProEo). (A) Co-incubation of 5 × 10⁻⁴ M or 10⁻³ M tetracycline hydrochloride (Tetra), doxycycline (Doxy), or minocycline (Mino) wit SEA, SEB, and SEC (5 μg/ml each; p < 0.05 up to p < 0.001 vs. medium control = 0). Data are presented as percentage of apoptotic nuclei ± SEM (n = 6). (B) Tetra partly abrogated the effect of ProEo. Mean percentage of apoptotic nuclei ± SEM (n = 5) after stimulation for 24 h (unfilled bars) and 48 h (striped bars). P < 0.05 up to p < 0.001.