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. 2022 Apr 1;12:5563. doi: 10.1038/s41598-022-09474-5

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

The workflow of the development of Targeted Amplicon Deep sequencing (TADs) for six drug resistance genes of P. falciparum by using Ion Torrent PGM and Illumina MiSeq protocol. There are three steps. Firstly, two TADs protocols were developed by using reference stain K1. Secondly, two TADs protocols were validated with P. falciparum strain K1 used as a positive control. Finally, two TADs protocols and conventional sequencing were performed in P. falciparum isolates from Ubon Ratchathani province and the SNPs results were compared.