Figure 3.
Macrophage liver kinase B1 (LKB1)–deficient mice show reduced cytokine release into the airways on lipopolysaccharide (LPS) challenge. Cytokine concentrations (tumor necrosis factor [TNF], CXCL1, and interleukin 1β, 10, and 6 [IL-1β, IL-10, and IL-6]) (A); numbers of total cells, alveolar macrophages (AMs) and neutrophils (B); and myeloperoxidase (MPO) concentration (C) measured in bronchoalveolar lavage fluid (BALF) 6 hours after intranasal inoculation with 1 µg LPS. Data are shown as box-and-whisker diagrams representing 8 mice per group. Cytokine and MPO levels of the LysM-cre × Stk11fl/fl mice were compared with those in littermate control (Stk11fl/fl) mice using the Mann-Whitney test. Cell counts were compared using the Student t test with Holm-Sidak correction for multiple testing. *P < .05; †P < .01.