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. 2020 Jul 10;225(7):1284–1295. doi: 10.1093/infdis/jiaa416

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© The Author(s) 2020. Published by Oxford University Press for the Infectious Diseases Society of America.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 4. — Macrophage liver kinase B1 (LKB1) deficiency is associated with the presence of “nonclassic” alveolar macrophages (ncAMs). A, Number of all alveolar macrophages (AMs) (CD45⁺, CD11c⁺, Ly6G⁻) in bronchoalveolar lavage fluid (BALF) from naive LysM-cre × Stk11^fl/fl mice and littermate controls (Stk11^fl/fl). B, CD11c, CD11b, and SiglecF expression of AMs. C, Fractional contribution of “classic” AMs (cAMs) (SiglecF^highCD11b^neg) and ncAMs (SiglecF^lowCD11b^pos) in BALF. Data are shown as box-and-whisker diagrams or as bar graphs showing mean percentage with standard error of the mean for 7–9 mice per group. Cell surface markers and cell numbers of the LysM-cre × Stk11^fl/fl mice were compared with those of littermate control (Stk11^fl/fl) mice, using the Student t test. ‡P < .001.