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. 2022 Mar 18;13:850037. doi: 10.3389/fimmu.2022.850037

Table 2.

Estimation of parameters in a multinomial logistic regression model for the classification of Trypanosoma cruzi (strain “Y”) load in the blood (undetected, light, moderate, and high) in wild-type C57BL/6 and knockout (iNOS-/-) mice.

Wild-type C57BL/6 mice
Parasitemia_classification Predictor (Parameter) Odds ratio 95% Confidence Interval p-value
Moderate - Light Lower Upper
BUN - mg/dL 1.0966 1.0964 1.0969 < .001
Plasma GOT - U/L 0.827 0.8191 0.835 < .001
Plasma ALP - U/L 0.4308 0.4304 0.4312 < .001
Total plasma protein - g/dL 0.8265 0.8265 0.8265 < .001
Plasma albumin - g/dL 0.7446 0.7446 0.7446 < .001
Plasma globulin - g/dL 0.8358 0.8358 0.8358 < .001
Plasma A/G ratio 1.1162 1.1161 1.1162 < .001
Urinary potassium - mmol/L 1.0413 1.0354 1.0473 < .001
Urinary urea - mg/dL 0.9872 0.9075 1.074 0.765
Erythrocytes - mm3 1 0.9999 1.0002 0.485
Hemoglobin - g/dL 0.6049 0.6049 0.605 < .001
Hematocrit - % 0.239 0.239 0.239 < .001
Total leukocytes - mm3 1.0738 1.0274 1.1222 0.002
Lymphocytes - mm3 0.9277 0.8874 0.9697 < .001
Monocytes - mm3 0.9135 0.9023 0.9249 < .001
TNF-α - pg/mL/g 1.0109 1.0036 1.0182 0.003
IL-10 - pg/mL/g 0.905 0.8965 0.9136 < .001
IFN-γ - pg/mL/g 1.0448 1.0355 1.0542 < .001
IL-12p40 - pg/mL/g 1.0504 1.0412 1.0596 < .001
IL-17 - pg/mL/g 1.1519 1.1422 1.1617 < .001
Cell number - mm2 0.9608 0.9472 0.9745 < .001
Not Detected - Light
BUN - mg/dL 0.0325 0.0325 0.0325 < .001
Plasma GOT - U/L 0.918 0.9091 0.927 < .001
Plasma ALP - U/L 0.8327 0.8319 0.8334 < .001
Total plasma protein - g/dL 0.997 0.997 0.997 < .001
Plasma albumin - g/dL 0.415 0.415 0.415 < .001
Plasma globulin - g/dL 1.0981 1.0981 1.0982 < .001
Plasma A/G ratio 0.9879 0.9879 0.9879 < .001
Urinary potassium - mmol/L 1.0883 1.0819 1.0947 < .001
Urinary urea - mg/dL 0.9902 0.9082 1.0795 0.823
Erythrocytes - mm3 1 0.9999 1.0002 0.514
Hemoglobin - g/dL 0.3925 0.3925 0.3925 < .001
Hematocrit - % 0.1281 0.1281 0.1281 < .001
Total leukocytes - mm3 1.0211 0.9754 1.0688 0.372
Lymphocytes - mm3 0.9746 0.9314 1.0198 0.266
Monocytes - mm3 0.9652 0.9532 0.9774 < .001
TNF-α - pg/mL/g 0.9633 0.9562 0.9706 < .001
IL-10 - pg/mL/g 0.9126 0.9038 0.9214 < .001
IFN-γ - pg/mL/g 1.0499 1.0407 1.0592 < .001
IL-12p40 - pg/mL/g 1.0892 1.0796 1.0989 < .001
IL-17 - pg/mL/g 1.1993 1.189 1.2097 < .001
  Cell number - mm2 0.9453 0.9319 0.959 < .001
Knockout mice (iNOS-/-)
Parasitemia_classification Predictor (Parameter) Odds ratio 95% Confidence Interval p-value
High – Not Detected   Lower Upper
Plasma GOT - U/L 1.020 1.002 1.036 0.030
Plasma ALP - U/L 0.965 0.901 1.035 0.319
Total plasma protein - g/dL 37.530 2.190 64.350 0.012
Plasma albumin - g/dL 0.012 0.008 0.650 0.031
Plasma globulin - g/dL 19.760 2.228 167.330 0.032
Plasma A/G ratio 0.030 0.005 14.080 0.699
Hemoglobin - g/dL 0.355 0.160 0.787 0.011
Neutrophils - mm3 1.002 0.998 1.004 0.076
Moderate-Not Detected
Plasma GOT - U/L 1.011 0.995 1.027 0.187
Plasma ALP - U/L 0.889 0.833 0.950 < .001
Total plasma protein - g/dL 4.860 0.385 61.280 0.222
Plasma albumin - g/dL 0.084 0.021 0.875 0.047
Plasma globulin - g/dL 42.150 0.575 309.450 0.076
Plasma A/G ratio 0.070 0.010 9.410 0.699
Hemoglobin - g/dL 0.274 0.109 0.692 0.006
  Neutrophils - mm3 1.002 0.995 1.003 0.149

BUN, blood urea nitrogen; GOT, glutamate oxaloacetate transaminase; ALP, alkaline phosphatase; A/G ratio, albumin to globulin ratio; TNF, tumor necrosis factor; IL, interleukin; IFN, interferon; Inf, infinite; Not detected = absence of parasites (by the method applied). Light = 50,000–200,000 parasites/mL. Moderate = 201,000–500,000 parasites/mL. High = above 501,000 parasites/mL. Odds ratio and 95% confidence interval = They indicate the estimate for the predictor effect size in a confidence interval associated with the blood parasite load outcome.