Figure 3.

Modeling kidney injury in human kidney organoids. (A) Fully differentiated day 25 kidney organoids created from hiPSCs on transwells. (B) Immunofluorescence of kidney organoids at day 25 shows the presence of various kidney cell types including collecting ducts (GATA3), distal tubules (ECAD), proximal tubules (LTL, PAX8, PAX2, SLC3A1, CUBILIN), and glomeruli (NEPHRIN). Cell nuclei were labeled with DAPI (blue). (C) Representative immunofluorescence of kidney organoid treated with 5 μM of cisplatin from day 21–23. Cisplatin treatment upregulated expression of kidney injury molecule KIM-1 (red) in the proximal tubule (LTL) compared with the control. GATA3, GATA binding protein 3; ECAD, epithelial cadherin; LTL, lotus tetragonolobus lectin; PAX2, PAX8, paired box homeotic gene 2 and 8. Scale bar 50 μm.