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. 2020 Jan 16;2(1):22–26. doi: 10.1097/BS9.0000000000000037

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Copyright © 2020 The Authors. Published by Wolters Kluwer Health Inc., on behalf of the Chinese Association for Blood Sciences.

This is an open access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND), where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal. http://creativecommons.org/licenses/by-nc-nd/4.0

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T lineage commitment from PSC-derived cell in 3D coculture systems. (A) In FTOC system, PSC are cocultured with OP9-DLL1 for mesoderm specification, hematopoietic induction, and then T cell progenitor induction. To avoid the regeneration of abnormal T lymphocytes, T cell progenitors are cultured with thymic lobes from E15.5 murine embryos. (B) In ATO system, mesoderm specification is initiated from PSCs on matrigel to produce CD326⁻CD56⁺ embryonic mesodermal progenitors (EMPs) that are cocultured with MS5-DLL1 or MS5-DLL4 as an embryonic mesodermal organoid (EMO) and artificial thymic organoid (ATO) system. Hematopoietic induction and T cell induction are induced by medium change at different stages.