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. Author manuscript; available in PMC: 2023 Mar 23.
Published in final edited form as: J Am Chem Soc. 2022 Mar 8;144(11):4839–4844. doi: 10.1021/jacs.1c12328

Figure 2.

Figure 2.

Precise determination of diffusivity via single-molecule statistics. (a) Normalized count distributions of the measured 600-µs single-molecule displacements for Cy3B-labeled catalase diffusing in PBS buffers containing 0%, 5%, 10%, and 15% glycerol. (b) The measured D as a function of the glycerol percentage, for two batches of samples measured a month apart (squares and circles; 95% confidence intervals are comparable to the symbol sizes), compared to that is predicted by scaling the 0% glycerol value with the known glycerol concentration-dependent viscosity (dash line). (c) SMdM-measured D values of 13 different protein samples of varying sizes in PBS and Tris buffers. Dash line: expected values at 20 °C according to the Young-Carroad-Bell model. See protein sample details in Table S1.