| Strain, strain background (Human cytomegalovirus) |
AD169 |
Hobom et al., J. Virol. 2000PMID:10933677
|
|
based on BAC HB15 |
| Strain, strain background (Human cytomegalovirus) |
TB40E |
Sinzger et al., J. Gen. Virol. 2008PMID:18198366
|
|
based on TB40E-Bac4 |
| Genetic reagent (Human cytomegalovirus) |
AD169ΔIE1 |
Scherer et al., J Virol. 2016PMID:26559840
|
|
based on BAC HB15 |
| Genetic reagent (Human cytomegalovirus) |
AD169/IE1-L174P |
Scherer et al., J Virol. 2016PMID:26559840
|
|
based on BAC HB15 |
| Genetic reagent (Human cytomegalovirus) |
TB40E-IE2-EYFP |
Wagenknecht et al., 2015PMID:26057166
|
|
based on TB40E-Bac4 |
| Genetic reagent (Human cytomegalovirus) |
TB40E-ΔIE1-IE2-EYFP |
this paper |
|
Deletion of IE1 from TB40E-IE2-EYFP |
| Cell line (Homo-sapiens) |
U2OS |
ATCC |
HTB-96 |
|
| Cell line (Homo sapiens) |
HEK293T |
DMSZ |
ACC 635 |
|
| Antibody |
mAb-PML G8 (Mouse monoclonal) |
Santa Cruz Biotechnology |
Cat# sc-377340 |
IF (1:500) |
| Antibody |
pAb-PML #4 (Rabbit polyclonal) |
Peter Hemmerich, Jena, Germany |
|
IF (1:500) |
| Antibody |
pAb-PML A301-167A (Rabbit polyclonal) |
Bethyl Laboratories |
Cat# A301-167ARRID: AB_873108
|
IF (1:1000),WB (1:5000) |
| Antibody |
pAb-PML A301-168A (Rabbit polyclonal) |
Bethyl Laboratories |
Cat# A301-168ARRID: AB_2284081
|
IF (1:1000),WB (1:5000) |
| Antibody |
pAb-Sp100 B01 (Mouse polyclonal) |
Abnova |
Cat# H00006672-B01, RRID:AB_982933
|
IF (1:1000) |
| Antibody |
pAb-Sp100 GH3 (Rabbit polyclonal) |
Hans Will, Hamburg, Germany |
|
WB (1:10000) |
| Antibody |
mAb-Daxx MCA2143 (Mouse monoclonal) |
Bio-Rad |
Cat# MCA2143, RRID:AB_2088900
|
IF (1:400) |
| Antibody |
pAb-ATRX H300 (Rabbit polyclonal) |
Santa Cruz Biotechnology |
sc-15408 (discontinued) |
IF (1:500) |
| Antibody |
Ab-HP1α 2,616 (Rabbit polyclonal) |
Cell Signaling Technology |
Cat# 2,616 |
IF (1:200) |
| Antibody |
pAb-SUMO2/3 ab22654 (Rabbit polyclonal) |
Abcam |
Cat# ab22654, RRID:AB_2198415
|
IF (1:500) |
| Antibody |
mAb-SUMO1 (Mouse monoclonal) |
Gerrit Praefcke, Langen, Germany |
|
IF (1:100) |
| Antibody |
pAb-phospho-Histone H2A.X 20E3 (Rabbit monoclonal) |
Cell Signaling Technology |
Cat# 9718, RRID:AB_2118009
|
IF (1:500) |
| Antibody |
mAB-TRF1 TRF78 (Mouse monoclonal) |
Santa Cruz Biotechnology |
Cat# sc-56807, RRID:AB_793407
|
IF (1:250) |
| Antibody |
mAb-IE1 p63-27 (Mouse monoclonal) |
Andreoni et al., J. Virol. Methods 1989PMID:2542350
|
|
WB (1:100), IF (1:5) |
| Antibody |
mAb-UL44 BS 510 (Mouse monoclonal) |
Plachter et al., Virus Res. 1992PMID:1329369
|
|
WB (1:5000), IF(1:1000) |
| Antibody |
mAb-MCP 28–4 (Mouse monoclonal) |
Waldo et al., Lancet 1989PMID:2463443
|
|
WB (1:2), IF (1:2) |
| Antibody |
pAb-IE2 pHM178 (Rabbit polyclonal) |
Hofmann et al., J Virol 2000PMID:10684265
|
|
WB (1:5000),IF (1:1500) |
| Antibody |
pAb-UL84 (Rabbit polyclonal) |
Hofmann et al., J Virol 2000PMID:10684265
|
|
WB (1:5000),IF (1:1500) |
| Antibody |
anti-FLAG (Mouse monoclonal) |
Sigma-Aldrich |
Cat# F1804, RRID:AB_262044
|
IF (1:1000) |
| Antibody |
anti-Myc (Mouse monoclonal) |
produced in hybridoma cells, ATCC |
MYC 1-9E10.2 [9E10] |
WB (1:10), IF (1:2) |
| Antibody |
anti-beta-Actin (Mouse monoclonal) |
Sigma-Aldrich |
Cat# A5441, RRID:AB_476744
|
WB (1:10000) |
| Antibody |
anti-phospho-STAT2 (Tyr689) |
Millipore |
Cat# 07–224 |
WB (1: 500) |
| Antibody |
anti-Interferon-alpha/beta Receptor Chain 2 (Mouse monoclonal) |
Millipore |
Cat# MAB1155, RRID:AB_2122758
|
(5 µg/ml) |
| Recombinant DNA reagent |
pLVX-shRNA1 (plasmid) |
Clontech |
|
lentiviral construct for stable knockdown |
| Recombinant DNA reagent |
pLVX-shPML (plasmid) |
Wagenknecht et al., 2015PMID:26057166
|
|
lentiviral construct for stable knockdown |
| Recombinant DNA reagent |
pLVX-shSp100 (plasmid) |
Wagenknecht et al., 2015PMID:26057166
|
|
lentiviral construct for stable knockdown |
| Recombinant DNA reagent |
pLVX-shDaxx (plasmid) |
Wagenknecht et al., 2015PMID:26057166
|
|
lentiviral construct for stable knockdown |
| Recombinant DNA reagent |
pLVX-shATRX (plasmid) |
this paper |
|
cloning of shRNA directed against ATRX into pLVX-shRNA1 |
| Recombinant DNA reagent |
pLKO-FLAG-PML I to VI (plasmid) |
Cuchet et al., J Cell Sci 2011PMID:21172801
|
|
lentiviral constructs for stable expression |
| Recombinant DNA reagent |
pInducer20-CRSmut (plasmid) |
Schilling et al., PLoS Pathog. 2021PMID:33770148
|
|
lentiviral construct for inducible expression, mutated cis-repression signal |
| Recombinant DNA reagent |
pInducer20-CRSmut-mCherry-PML VI (plasmid) |
this paper |
|
lentiviral construct for inducible expression, mutated cis-repression signal |
| Recombinant DNA reagent |
pLKO-Myc-IE1 (plasmid) |
Scherer et al., PLoS Pathog 2014PMID:25412268
|
|
lentiviral construct for stable expression |
| Recombinant DNA reagent |
pLKO-Myc-IE1 1–382 (plasmid) |
Scherer et al., PLoS Pathog 2011PMID:26559840
|
|
lentiviral construct for stable expression |
| Chemical compound, drug |
Camptothecin; CPT |
Santa Cruz Biotechnology |
Cat# sc-200871 |
|
| Chemical compound, drug |
Doxorubicin hydrochloride; Doxo |
Cayman Chemical |
Cat# Cay15007 |
|
| Chemical compound, drug |
ATM inhibitor KU 55933; ATMi |
Tocris Bioscience |
Cat# 3,544 |
|
| Chemical compound, drug |
5-Ethynyl-2′-deoxycytidine; EdC |
Sigma-Aldrich |
Cat# T511307 |
|
| Chemical compound, drug |
5-Ethynyl-2′-deoxyuridine; EdU |
Sigma-Aldrich |
Cat# 900,584 |
|
| Chemical compound, drug |
(2′S)–2′-Deoxy-2′-fluoro-5-ethynyluridine; F-ara-EdU |
Sigma-Aldrich |
Cat# T511293 |
|
