Figure 3. Auxin-inducible gene expression system (AGES) allows induction of GAL4 activity in Drosophila larvae.

(A) GFP fluorescence quantification in fat body tissue after induction (for 24 hr) on food with different concentrations of auxin. The average pixel intensities from six larvae were quantified and analysed using Kruskal-Wallis test with Dunn pair-wise comparison (***, p < 0.001 and **, p < 0.01). (B) Time course of GFP expression in fat body tissue when using 5 mM auxin. The average pixel intensities from six larvae were quantified and analysed using Kruskal-Wallis test with Dunn pair-wise comparison (**, p < 0.01). (C) Representative images of larvae fed on different concentrations of auxin. (D) Representative images of larvae imaged at each time interval since induction. See Figure 3—source data 1 for raw data.