Skip to main content
. 2022 Apr 1;11:e67598. doi: 10.7554/eLife.67598

Figure 4. Auxin-inducible gene expression system (AGES) allows induction of GAL4 activity in the Drosophila adult and larval brain.

(A) GFP fluorescence driven by Or85a-GAL4 in the antennal lobe, with and without auxin. (B) GFP fluorescence quantification in the antennal lobe 24 hr after induction (four to six replicates). Statistics performed using ordinary one-way ANOVA (*, p < 0.05). (C) GFP fluorescence driven by grh-GAL4 in the larval ventral nerve cord, with and without auxin. (D) GFP fluorescence quantification in the ventral nerve cord 24 hr after induction (six replicates). Statistics performed using ordinary one-way ANOVA (*, p < 0.05). See Figure 4—source data 1 for raw data.

Figure 4—source data 1. GFP intensity values for Figure 4.

Figure 4.

Figure 4—figure supplement 1. Auxin-inducible gene expression system (AGES) allows induction of pan-neuronal GAL4 activity in the adult brain.

Figure 4—figure supplement 1.

Confocal images of adult brains stained with anti-GFP and anti-Elav. Adults were fed food containing 5 mM auxin for 24 hr. Scale bars represent 20 µm.