FIG. 1.

FK506 and L-685,818 promote FKBP12 binding to cryptococcal calcineurin. Binding assays with purified FKBP12 immobilized on Affi-Gel beads were performed with equal amounts of protein extract from serotype A frr1::ADE2 CNA1 strain MCC1, in the absence (−) or presence of 20 μM FK506 and L-685,818. The migration position of the Cna1 protein is indicated by an arrow. Total protein extracts from serotype A CNA1 wild-type and cna1 mutant strains served as controls for the identity and electrophoretic mobility of the calcineurin A catalytic subunit Cna1.