TABLE 1.
Plasmids and strains used in this study.
| Plasmids/Strains | Description | Source |
|---|---|---|
| Plasmids | ||
| pESC-URA | AmpR, URA3, 2-micron ori | Agilent |
| pUG72 | AmpR, loxP–URA3–loxP | Gueldener et al. (2002) |
| pUG72-TEF1 | pUG72 carrying PTEF1 | Yu et al. (2016) |
| pSH69 | AmpR, hpnMX, Cre under PGAL1 control | Hegemann and Heick, (2011) |
| pPDR12-GFP | pESC-URA carrying PPDR12 and yEGFP | This study |
| Strains | ||
| E. coli | ||
| E. coli TOP10 | F− mcrA Δ (mrr-hsdRMS-mcrBC) ϕ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL(Str R ) endA1 nupG | Invitrogen |
| S. cerevisiae | ||
| BY4741 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 | ATCC |
| 4G-ΔADH6 | BY4741 with overexpressed BAT1, ARO10, ALD2, ALD5 and disrupted ADH6 | Yu et al. (2016) |
| TOE01 | BY4741 with overexpressed PDR12 | This study |
| TDL01 | BY4741 with disrupted PDR12 | This study |
| TDL02 | 4G-ΔADH6 with disrupted PDR12 | This study |
| BY4741-SLCA | BY4741 with pPDR12-GFP | This study |
| 4G-ΔADH6-SLCA | 4G-ΔADH6 with pPDR12-GFP | This study |
| TOE01-SLCA | TOE01 with pPDR12-GFP | This study |
| TDL01-SLCA | TDL01 with pPDR12-GFP | This study |
| TDL02-SLCA | TDL02 with pPDR12-GFP | This study |