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. 2022 Mar 25;2022:7508928. doi: 10.1155/2022/7508928

Figure 7.

Figure 7

Evaluation of myeloid cell subpopulation in tumor-draining lymph nodes after administration of vaccine cells. (a) Scheme of the multiparameter flow cytometric analysis of DC and Mf on the 3rd, 5th, and 7th days after single injection of DCs in tumor-draining lymph nodes. The analysis was started by eliminating debris and cell aggregates; then, live leukocytes (DAPI−CD45+) were gated, and among them, CD11b+ myeloid cells were identified. The CD11b+ cells were subsequently separated for expression of the CD11c+ marker. Among the CD11c+ cells, the DC (CD11c+F4/80int) and TAM (CD11c+F4/80+) populations were identified. (b) Percentage of CD11b+ cells in lymph nodes. (c) Percentage of DCs in lymph nodes. (d) Percentage of Mf in lymph nodes. Results are presented as mean + SD calculated for 5-6 mice per group. Differences between groups were estimated using the parametric Brown-Forsythe and Welch ANOVA test followed by Dunnett's T3 multiple comparison post hoc test ((c) percentage of DC cells on the 5th day) or the nonparametric Kruskal-Wallis test followed by Dunn's multiple comparison post hoc test ((d) percentage of Mf on the 5th day) (∗p < 0.05, ∗∗p < 0.01).