Figure 2.

Effect of LEF1-AS1 on cell proliferation and apoptosis. (a, b) Efficiency of LEF1-AS1 overexpression plasmid (a) or LEF1-AS1 siRNAs (b) in FaDu cells. (c, d) GSEA analysis of P53 (c) and apoptosis (d) gene sets based on LEF1-AS1 expression information in TCGA. (e, f) CCK8 analysis in LEF1-AS1 overexpressed (e) or silenced FaDu cells (f). (g) Colony formation assay. (h) Relative colony numbers in (g). (i) Apoptosis of LEF1-AS1 overexpressed or silenced FaDu cells were detected by flow cytometry. (j) Apoptotic cell percent in (i). (k) Protein levels of apoptosis markers including cleaved caspase 3, Bax, and Bcl-2 in LEF1-AS1 overexpressed or silenced FaDu cells. Data are presented as means ± SD. ∗∗p < 0.01; ∗∗∗p < 0.001.