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. 2022 Feb 19;41(14):2095–2105. doi: 10.1038/s41388-022-02223-y

Fig. 1. TSPAN6 controls epithelial cell migration and cooperates with H-RASV12 to induce cell invasion in normal human mammary epithelial cells.

Fig. 1

a The knockdown of SCRIB, DLG1, and TSPAN6 leads to decreased epithelial sheet migrative potential. Representative phase contrast microscope images of Scrib, Dlg1, or TSPAN6 knockdown MCF10A epithelial cell monolayers at 0, 12, and 18 h after wounding, and quantification of wound closure from 0–24 h. In each case, the corresponding shRNA constructs had a slower wound closing time as compared to the Scramble shRNA control (SCRIB sh vs Scram P < 0.0001; DLG1 sh vs Scram P < 0.0001; TSPAN6 sh vs Scram P < 0.0001; two-way ANOVA, 3 independent experiments). Scale bar = 100 μM. b SCRIB, DLG1 and TSPAN6 knockdown promote H-RASV12-induced invasion in 3D cultures. In combination with low or high levels of H-RASV12, knockdown of SCRIB, DLG1, and TSPAN6 led to increased invasive acini in 3D culture. (*P < 0.0001, ** P < 0.01, ***P < 0.05; one-way ANOVA, three independent experiments). See Supplementary data for microscope images.