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. 2022 Feb 19;41(14):2095–2105. doi: 10.1038/s41388-022-02223-y

Fig. 3. TSPAN6 inhibits growth and metastasis of activating RAS mutant human epithelial tumor cells.

Fig. 3

a Schematic vector map of doxycycline (Dox) inducible mCherry-IRES-TSPAN6. Bottom panel shows doxycycline-inducible TSPAN6 protein expression in stably transfected MIA PaCa2 and PANC1 cells. b Ras activation assay measured upon EGFα stimulation in PANC1 in the absence of or upon induced TSPAN6 expression. Experiments were performed in six repeats. c Transwell migration of TSPAN6-PANC1 cells in response to treatment with EGFα and/or growing FBS gradient. For each condition, experiments were carried out in triplicate. d Representative bioluminescence images of nu/nu recipient mice orthotopically transplanted with the Dox-inducible TSPAN6 human pancreatic cancer cell lines MIA PaCa2 (upper panels) and PANC1 (lower panels) in the presence or absence of Dox. e Representative H&E staining of liver and diaphragm sections of TSPAN6 ON and TSPAN6 OFF PANC1 cohorts 12 weeks after orthotopic grafting, showing reduction of metastatic foci (arrows) upon TSPAN6 expression. Scale bars = 200 μM. f Representative intestinal sections (H&E staining) of mice following orthotopic injection of TSPAN6 ON and TSPAN6 OFF PANC1 cells. Scale bars = 500 μM.