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. 2022 Feb 19;41(14):2095–2105. doi: 10.1038/s41388-022-02223-y

Fig. 4. TSPAN6 Interacts with the EGFR but not with SCRIB or DLG1.

Fig. 4

a Co-immunoprecipitation between TSPAN6 and the EGFR. PANC1 or MIA-PaCa2 cells were lysed in 1% Brij 98 protein extraction buffer. After immunoprecipitation (IP) with anti-EGFR or IgG control antibodies, proteins were separated by SDS-PAGE and detected by immunoblotting with anti-TSPAN6 or anti-EGFR antibodies (IB). b Co-immunoprecipitation between FLAG-TSPAN6 overexpressing pancreatic cell lines and the EGFR. PANC1 or MIA-PaCa2 cells were lysed in 1% Brij 98 protein extraction buffer. After immunoprecipitation (IP) with anti-FLAG or IgG control antibodies, proteins were separated by SDS-PAGE and detected by immunoblotting with anti-EGFR, anti-SCRIB, anti-DLG1, or anti-FLAG antibodies (IB) The Lysate is also shown. Experiments were conducted once.