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. 2022 Feb 19;41(14):2095–2105. doi: 10.1038/s41388-022-02223-y

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© Crown 2022, corrected publication 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Kaplan–Meier survival plots for whole-body Tspan6^-/y Kras^G12D mice (n = 8) and TSPAN6^+/y Kras^G12D (n = 9) littermate controls. Log-rank test was used for statistical analysis. **P < 0.01. b Representative histological H&E sections of Tspan6^-/y Kras^G12D and Tspan6^+/y Kras^G12D lungs 12 weeks after Ad-Cre infection. Magnifications x5. c Quantification of hyperplasic regions, adenomas, and adenocarcinomas in Tspan6^-/y Kras^G12D and Tspan6^+/y Kras^G12D lungs at the indicated time points following Ad-Cre inhalation. n = 5 for each cohort and time point analyzed. Data are shown as means ± s.e.m. * P < 0.05; ** P < 0.01; N.S. = not significant (Student’s t test). d Representative in situ immunostaining for phosphorylated EGFR (pEGFR) 8 weeks post Ad-Cre delivery to lungs of Tspan6^-/y Kras^G12D (n = 5) and Tspan6^+/y Kras^G12D mice (n = 5). Right panel shows quantification of % pEGFR⁺ cells in hyperplasic regions, adenomas, and adenocarcinomas. Data are shown as mean ± s.e.m. * P < 0.05; ***P < 0.001; N.S. = not significant (Student’s t test). Scale bars = 200 μM. e Representative immunostaining for the mesenchymal markers, vimentin, and N-cadherin, and the epithelial marker E-cadherin in lung tumors 8 weeks after Ad-Cre inhalation. Sections were counterstained with haematoxylin. Scale bars, top left panel = 100 μM, bottom left panel = 200 μM, middle panels = 200 μM, right panels = 500 μM.