Extended Data Fig. 6. GO cluster representation in co-growing auxotrophs vs prototrophs.
GO cluster and Gene Set Enrichment Analysis (GSEA) using the proteomics data from the sorted auxotrophic and prototrophic subpopulations, derived from single-plasmid CFP SeMeCos. Data are from n = 16 independent SeMecos sorting experiments (n = 4 independent experiments for each single-plasmid complemented strains (pH-, pL-, pU- and pM-SeMeCos). P-values obtained from GSEA were used to calculate a simRel score, a functional similarity measure for comparing two GO terms with each other and projected onto two dimensional space (x and y-axes) derived by applying multidimensional scaling to a matrix of the GO terms’ semantic similarities that generates slimmed GO terms. Left and right columns indicate upregulated and downregulated proteins respectively in auxotrophs when compared to the corresponding prototroph. Significance threshold is defined as P < 0.05. Bubble colour indicates log10(P-value), with blue being highly significant and red being less significant. Bubble sizes indicate gene ratio, which is the frequency of representation of the GO term within the Uniprot database for S. cerevisiae. A larger circle would indicate a more general term compared with a smaller circle that indicates a more specific term. All plots were generated via REVIGO4.