Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Apr 1;13:1731. doi: 10.1038/s41467-022-29420-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 2 — a Western blot analysis of BRG1 in whole-cell extracts prepared from HCT116 and BRG1-deficient (KO) clone 2 cells at early, mid (4 months) and late (8 months) time points. α-tubulin was used as a loading control. Similar results were obtained in three independent repeats. b The proliferation rate of BRG1 knockout clone 2 improves during long-term cell culture. Cell number was monitored every 2 days over a period of 9 days in HCT116 and BRG1 KO clone 2 cells taken from early, mid (4 months) and late (8 months) time points. Data were presented as the mean ± SD; n = 3. The p value was calculated with two-way ANOVA-Tukey. **p < 0.01, ***p < 0.001. c The colony size of BRG1 knockout clone 2 increases during long-term cell culture. Clonogenic assay showing the colony size of HCT116 (BRG1wt) and BRG1 knockout (KO) cells taken from early, mid (4 months) and late (8 months) time points. d The cell cycle profile of BRG1-deficient cells changes during long-term cell culture to a profile similar to parental HCT116 cells. Representative flow cytometry profiles in HCT116 and BRG1-deficient cells (clone 2) at early and late (8 months) time points. e Quantification of cells (%) in each cell cycle phase from flow cytometry profiles in HCT116 and BRG1 knockout clone 2 from early and late (8 months) time points. Data were presented as the mean ± SD; n = 3. The p value was calculated with two-way ANOVA-Tukey. *p < 0.05, **p < 0.01.