Table 3.
Cell proliferation. Effect of lysophosphatidic acid (LPA) 18:1 and the LPA receptor 1, 3 (and 2) inhibitor Ki16425 on cell proliferation after 24 h, 48 h and 72 h of different human breast cancer cell lines. LPA stimulated significantly proliferation of MDA-MB-231 and MDA-MB-468 cells, and trended to stimulate proliferation of MCF-7 cells.
| Group | MDA-MB-231 | MDA-MB-468 | MCF-7 | BT-474 | SKBR-3 | MCF-10A |
|---|---|---|---|---|---|---|
| LPA 0.1 μM | + | + | + | Ø | Ø | Ø |
| LPA 1.0 µM |
++ p = 0.03 (72 h) |
++ p = 0.05 (72 h) |
+ | Ø | Ø | Ø |
|
LPA 0.1 μM + Ki16425 2 μM |
Ø | Ø | Ø | Ø | Ø | Ø |
|
LPA 1.0 μM + Ki16425 2 μM |
Ø | Ø | Ø | Ø | Ø | – |
|
LPA 0.1 μM + Ki16425 20 μM |
Ø | Ø | Ø | Ø | Ø | Ø |
|
LPA 1.0 μM + Ki16425 20 μM |
– | – | – | Ø | Ø | – |
n = 3 replicate experiments, a p-value ≤ 0.05 was considered significant (Kruskal–Wallis test and post-hoc Dunn’s test).
Ø: no effect; +: stimulatory trend; ++: statistically significant stimulation; –: inhibitory trend. For LPA 0.1 μM and LPA 1.0 μM, stimulation was compared to the control group; for LPA 0.1 μM/LPA 1.0 μM + Ki16425 2/20 μM inhibition was compared to the respective LPA group without Ki16425.