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. 2022 Apr 1;5:300. doi: 10.1038/s42003-022-03214-1

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Comparison of cellular levels of SPT5 and p-SPT5 Thr-806 by Western blot in control and TOX4 KO cells. Top: Western blot images with nonspecific bands highlighted by a “*”, Bottom: A bar graph showing relative levels of p-SPT5 Thr-806 quantified by ImageJ in control and TOX4 KO cells. Pictures are representative of four independent experiments. Statistical significance was determined with a two-sided Student’s t-test; the centers and the error bars represent the mean and the SD, respectively. NS: P ≥ 0.05, *P < 0.05, **P < 0.01. b–d Correlation plots for biological replicates of CUT&Tag of SPT5 (b), p-SPT5 Thr-806 (c), and NELF-E (d). e Normalized CUT&Tag read distribution of Pol II, SPT5, p-SPT5 Thr806 and NELF-E within the SERBP1 locus in TOX4 KO versus control cells. f–h Genome-wide meta-gene profiles and heatmaps of CUT&Tag comparing chromatin occupancies of SPT5 (f), p-SPT5 Thr-806 (g), and NELF-E (h) in TOX4 KO versus control cells. Genes were sorted by total Pol II CUT&Tag signal in control cells.