Fig. 1. Effects of acute cocaine administration on CB₂ mRNA expression in the brain and spleen.

a A diagram showing the transcript structure of CB_2A isoform and two Taqman probes that target either the upstream region (mCB_2A probe) or the gene-deleted region (mCB₂-KO probe) in the CB₂-KO mice used in the present study. b, c qRT-PCR results, indicating that both the mCB_2A and mCB₂-KO probes detected similar levels of CB₂ mRNA in the striatum and spleen in WT mice. While the mCB_2A probe also detected similar levels of CB₂ mRNA in CB₂-KO mice, the mCB₂-KO probe detected CB₂ mRNA only in WT, not in CB₂-KO mice. d, e qRT-PCR results: indicating that a single injection of cocaine (10, 20, 30 mg/kg, i.p.) did not alter CB₂ mRNA expression, measured by both the mCB₂ probes, in either the PFC or striatum in WT mice as measured 1 h after acute cocaine injection.