Fig. 3. Brca2^L2431P mutant MEFs exhibit genomic instability.

a Growth curve of MEFs of different genotypes over 8 days. LP/LP and LP/KO MEFs have reduced relative growth compared to controls (n = 3 biological replicate, one-tailed t-test: two-sample assuming unequal variances, error bar- SE of mean). b Cell cycle analyses of MEFs of indicated genotypes shows LP/LP and LP/KO MEFs get stalled in G2 phase (n = 3 biological replicate, two-tailed Student’s t test, error bar- SD of mean). c Chromosomal aberrations in MEFs of indicated genotype, untreated (top panels) and after 100 nM MMC treatment (lower panels). Aberrations are marked with red arrows and radials are circled (scale bar = 5 μm). d Quantification of chromosomal aberrations in MEFs shown in c. LP/LP and LP/KO MEFs exhibit increased number of chromosomal aberrations after treatment as compared to WT (n = 3 individual MEFs with 15 nuclei each making 45 nuclei in total, two-tailed Student’s t test, error bar- SD of mean).