Table 3.
Expansion system of NK cell derived from iPSC or ESC
| Feeder cells NK: feeder cells | Medium | Culture time | Expansion fold | Purity of NK | Ref. | |
|---|---|---|---|---|---|---|
| initial | Expanded | |||||
| K562-CD64-CD86-41BBL-CD19-mbIL21 D0 1:2; Weekly 1:1 | RPMI 1640 + 10% FBS + 50 IU/ml rhIL-2 + l-glutamine + P/S | 6 weeks | >108-fold | ~10% | ~80% | [159] |
| K562-CD64-CD86-41BBL-CD19-mbIL21 D0 1:2; Weekly 1:1 | RPMI 1640 + 15% FBS + 1% P/S + 50 U/ml IL-2 | >6 weeks | 102~103-fold | 70-95% | >99% | [183] |
| K562-CD64-CD86-41BBL-CD19-mbIL21 D0 1:2; Weekly 1:1 | RPMI 1640 + 10 % FBS + 2 mM l-glutamine + 1 % P/S + 50 U/mL IL-2 | >6 weeks | >103-fold | No mentioned | No mentioned | [240, 243] |
| K562-CD64-CD86-41BBL-CD19-mbIL21 D0 1:2; Weekly 1:1 | RPMI 1640 + 10 % FBS + 2 mM l-glutamine + 1 % P/S + 50 U/mL IL-2 | No mentioned | a several log-fold | >97% | >97% | [184] |
| K562-CD64-CD86-41BBL-CD19-mbIL21 D0 1:2; Weekly 1:1 | 60% DMEM + 30% Ham’s F12 + 10% human AB serum + 20 µM β-ME + 50 µM ethanolamine + 20 µg/ml ascorbic Acid + 5 ng/ml sodium selenite + 10 mM HEPES + and 100–250 IU/ml IL-2 | No mentioned | No mentioned | No mentioned | No mentioned | [189] |
| K562-CD64-CD86-41BBL-CD19-mbIL21 D0 1:2; Weekly 1:1 | RPMI 1640 + 10 % FBS + 2 mM l-glutamine + 1 % P/S + 50 U/mL IL-2 | 4 weeks | >103-fold | 95% | No mentioned | [164, 182] |
| K562-CD64-CD86-41BBL-CD19-mbIL21 D0 1:2; Weekly 1:1 | RPMI 1640 + 15% FBS + 1% P/S + 50 U/ml IL-2 | No mentioned | No mentioned | No mentioned | No mentioned | [131, 145] |
| K562-CD64-CD86-41BBL-CD19-mbIL21 D0 1:2; Weekly 1:1 | RPMI 1640 + 2 mM l-glutamine + 1% P/S + 1% nonessential amino acids + 10% standard FBS or 10% human serum AB + 50-100 U/mL IL2 | No mentioned | No mentioned | No mentioned | No mentioned | [245] |
| K562-mbIL15-41BBL cells 1:10 | No mentioned | 2 weeks | 74-fold | No mentioned | No mentioned | [179] |
| irradiated PBMC No mentioned | No mentioned | 2 weeks | 66-fold | No mentioned | No mentioned | [113] |
mbIL21 membrane-bound IL-21, P/S penicillin–streptomycin, β-ME β-mercaptoethanol.