Fig. 4. Sensitivity of S4 residues to (R)-L3 modulation.
a–d Representative current traces of wildtype (a) and mutant channels L236A (b), R237A (c) and H240 (d). e Current amplitude activation of Kv7.1 channel mutants by 1 µM (R)-L3 expressed as percent change in current measured at the end of a 7-s pulse to +40 mV (n = 30 for WT, n = 13 for I235A, n = 18 for L236A, n = 12 for R237A, n = 18 for M238A, n = 13 for L239A, n = 12 for H240A, n = 16 for V241A). Significance of mean differences was evaluated by one-way ANOVA and posthoc mean comparison Tukey Test (*p < 0.05, **p < 0.01, ***p < 0.001). f–i Effect of (R)-L3 on hKv7.1 channel mutant activation and deactivation rates. Kinetics were evaluated in both absence and presence of 1 µM (R)-L3 and fitted by two-component exponential function (number of independent oocytes for (f–i) are given in SI Table 3).