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. 2021 Jul 16;43(4):829–839. doi: 10.1038/s41401-021-00727-z

Fig. 1. SFN increases Nrf2 and decreases MeCP2 expression, changing abnormal BDNF expression in stressed mice.

Fig. 1

a Chemical structure of Sulforaphane (SFN). b Schedule of behavior test and treatment. c The social interaction test for no target and target time (Mean ± SEM, n = 8 per group, one-way ANOVA, *P < 0.05, ***P < 0.001). d Locomotion test (Mean ± SEM, n = 9 per group, one-way ANOVA, *P < 0.05 and **P < 0.01). e Forced swimming test (Mean ± SEM, n = 9 per group, one-way ANOVA, **P < 0.01). f The sucrose preference test (Mean ± SEM, n = 8 per group, one-way ANOVA, **P < 0.01). g Western blot analysis of Nrf2, MeCP2, and BDNF in the mPFC (Mean ± SEM, n = 4 per group, one-way ANOVA, *P < 0.05, **P < 0.01). h Representative images and quantification of Nrf2 staining in the mPFC. Scale bar, 50 μm. (Mean ± SEM, n = 4 per group, one-way ANOVA, **P < 0.01, ***P < 0.001). i Representative images and quantification of MeCP2 staining in the mPFC. Scale bar, 50 μm. (Mean ± SEM, n = 4 per group, one-way ANOVA, **P < 0.01, ***P < 0.001). j SFN changes synaptic plasticity impairments in the mPFC of stressed Thy1-YFP mice. Representative photomicrographs for high magnification of spine density in the mPFC (Mean ± SEM, n = 6 per group, one-way ANOVA, **P < 0.01). Scale bar, 10 μm.