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. 2021 Jul 27;43(4):908–918. doi: 10.1038/s41401-021-00706-4

Fig. 5. High dose of pirfenidone was more effective in ameliorating silica-induced pulmonary fibrosis in advanced silicosis.

Fig. 5

Masson staining, Sirius red staining and immunohistochemistry (a) were performed in mouse lung tissues to evaluate the levels of pulmonary fibrosis. The fibrotic scores (b) were calculated based on Masson staining. The positive areas of fibrosis (c) were calculated based on Sirius red staining. The positive areas of collagen I (d) were calculated based on immunohistochemistry. The contents of hydroxyproline (e) in lung tissues were detected by an HYP kit. The mRNA expression levels of collagen I (f) and fibronectin (g) were tested by qPCR. The protein levels of fibronectin (h) were detected by Western blot analysis. All data were presented as mean ± SD; n = 10 for each group; *P < 0.05, ***P < 0.001, ****P < 0.0001.