Fig. 5. Exercise increases the level of TFEB in the nucleus and upregulates the expression of lysosomal genes.

a TFEB localization in mice cortex by immunofluorescent staining. b Total TFEB protein level was measured by Western blotting. c, d Western blot analysis of TFEB nuclear translocation by nuclear-cytoplasmic fractionation. GAPDH was used as a cytoplasmic loading control and histone H3 was used as a nuclear loading control (n = 3). e Analysis of the transcription level of lysosomal-associated genes regulated by TFEB by quantitative PCR (n = 7). Data represent means ± SD. *P < 0.05. **P < 0.01, ^ns P > 0.05 versus the control group. One-way ANOVA test.