Fig. 3.

D. maritima RbcL rosewood toehold switches. A. Schematic representation of the RbcL gene and the localisation of switches and triggers. B. In vivo characterization of sfGFP expression by E. coli BL21 Star™(DE3) cells harboring the DmRbcL toehold switches and triggers (Fig. 1C). The negative controls have been performed with an empty pSB3T5, pSB1C3 (no trigger) and BBa_K3453103 (no promoter, no RBS) and the positive controls with BBa_K3453104 and BBa_K3453105. The data and error bars are the mean and standard deviation of at least three measurements on independent biological replicates. C. MEFL/Particle fold changes of the DmRbcL toehold switches in the presence of the DmRbcL triggers compared to the MEFL/Particle value in the absence of the trigger.