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Pre-processing of CyTOF data
PBMCs from four healthy donors were stained with a panel of metal-labeled antibodies and analyzed by CyTOF. The resulting data was then normalized using Premessa. Manual gating was then used to remove EQ beads (140Ce high events) and exclude cell debris (193Ir-DNA2- cells) and dead cells (195Pt Cisplatin high cells) before exploratory data analysis was performed.
