PBMC media

Reagent	Final concentration	Amount
RPMI-1640	N/A	440 mL
HI-FBS	10% (v/v)	50 mL
Penicillin-streptomycin	100 U/mL penicillin and 100 μg/mL streptomycin	5 mL
200 mM L-glutamine	2 mM	5 mL
Total	N/A	500 mL

Store at 4°C for up to one month.