Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Apr 2;21:93. doi: 10.1186/s12943-022-01537-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 1 — Selection and validation of circMAP3K4. A Flowcharts showing the selection of circRNAs overexpressed in HCC with coding potential. B CircMAP3K4 expression in HCC and liver tissues from the CIRCpedia database. Tumor samples, n = 13; liver samples, n = 8. C CircMAP3K4 expression in paired HCC and adjacent liver tissues from the CIRCpedia database (n = 6). D The location of circMAP3K4 in the human genome (upper panel), and circMAP3K4 sketch map. CircMAP3K4 expression detected by RT-qPCR followed by Sanger sequencing using divergent primers. E CircMAP3K4 interspecies sequence conservation. Homo sapiens, Human; Gorilla gorilla, Gorilla; Macaca mulatta, Macaque; Bovini, Bovine; Mus musculus, Mouse; Pan troglodytes, Chimpanzee; Rattus norvegicus, Rat. F–H RT–qPCR analysis of circMAP3K4 expression after amplification with oligo (dT)18 primers, RNase R treatment and actinomycin D treatment, respectively. I CircMAP3K4 distribution detected by cytoplasmic and nuclear RNA fractionation. GAPDH and U3 were employed as positive controls in the cytoplasm and nucleus, respectively. J Fluorescence in situ hybridization showed the predominant cytoplasmic distribution of circMAP3K4 (Scale bar, 20 μm). All experiments were repeated at least three times. Data are shown as mean ± standard deviation (SD), * p < 0.05, ** p < 0.01, and *** p < 0.001 in two-way ANOVA (E) and t-test (C, D). cMAP3K4, circMAP3K4; mMAP3K4, MAP3K4 mRNA