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. 2022 Apr 2;41:122. doi: 10.1186/s13046-022-02345-x

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Hypoxic TME increases Cis-Pt resistance and CA IX/XII expression levels in HNSCC. A-B Cell viability analysis by MTT assay of FaDu and SCC-011 cells grown in normoxic (21% O₂) and hypoxic (1% O₂) conditions and incubated with increasing concentrations of Cis-Pt (0–10 µM) for 72 h. All the data are expressed as percentage of viable cells, considering the untreated control cells as 100%. Bars depict mean ± SD of three independent experiments. (***p < 0.001; **p < 0.01; *p < 0.1). C HIF-1α, CA IX and CA XII protein levels were analyzed by western blot analysis in FaDu and SCC-011 cell lines grown under normoxia (21% O₂) and hypoxia (1% O₂) for 72 h. Actin and GADPH were used as loading control. Representative data from one of three experiments are shown. D For CA IX and CA XII mRNA expression analysis in HNSCC patients the Genomics Analysis and Visualization platform (R2: Genomics analysis and visualization platform; http://r2.amc.nl) was used. The analysis was performed with the following datasets: GSE18674 which includes 22 human normal tissues and GSE42743 which includes 103 oral cavity tumors. The correlation was assessed by one-way analysis of variance (ANOVA), through the R2 platform and presented in box plots. (***p < 0.0001)