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. 2021 Oct 15;31(7):1035–1050. doi: 10.1093/hmg/ddab301

Figure 1.

Figure 1

Design of the Kv8.2 KO strain. (A) Kv8.2 (red) requires assembly with Kv2.1 (gray) to form a functional channel. Kv8.2 has the typical topology of a voltage-gated K+ channel subunit; a T1 tetramerization domain, voltage sensor and pore-forming domain. Guide RNAs were designed to excise 101 nt from Kcnv2 to generate a frameshifting truncation in the N-terminus of Kv8.2 prior to any functional domains. (B) Representative western blots of retina lysates from WT versus KO animals, probed for Kv8.2, Kv2.1, HCN1 and Na/K-ATPase (NKA). (C) Expression levels of proteins as measured in (B) normalized to WT levels. (D) Immunostaining for Kv8.2 (magenta) and Kv2.1 (green) in WT versus Kv8.2 KO photoreceptors. Abbreviations are OS, outer segment; IS, inner segment; ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer, and the scale bar is 20 μm.