Table 5. Primers for target and reference genes of Octopus maya oviducal glands used in RT-qPCR.
| Transcript | Protein name | E | Tm | Size | Primers |
|---|---|---|---|---|---|
| TRINITY_DN22438_c0_g1_i1 | 3-oxo-5-alpha-steroid 4-dehydrogenase 1 | 109.8 | 60 | 119 | CGGAAACCTAACGAAACAGG |
| GACCAGCATGCAATAGCAAA | |||||
| TRINITY_DN16362_c4_g6_i1 | Chorion peroxidase | 97.3 | 60 | 116 | CGGCTTATCACGACGGTTAT |
| GCATTTTGCGTTGAAAGGTT | |||||
| TRINITY_DN14910_c0_g1_i2 | Diamine acetyltransferase 2 | 90.5 | 60 | 127 | ACCCACCTTCTGTTGATGATCT |
| ACCAATGGTCCTGTGCTTAGT | |||||
| TRINITY_DN12903_c1_g1_i1 | Dopamine receptor 1 | 91.4 | 60 | 120 | GGCTGTGACCTCGACATCAA |
| GTGTTGCTGAGCCGTACTGT | |||||
| TRINITY_DN12984_c0_g1_i1 | Matrix metalloproteinase-19 | 94 | 60 | 150 | TGACGAAGAATGGACTGCAA |
| TGGAAATCCTTCACGAAACC | |||||
| TRINITY_DN32740_c0_g1_i1 | Myomodulin neuropeptides 1 | 97.2 | 62 | 118 | GCAGTGGACCATTCCTTGAT |
| TTTTCGAAGCCATTTTGTCC | |||||
| TRINITY_DN16150_c1_g1_i4 | Peroxiredoxin-4 | 100.9 | 60 | 107 | ATGGCCAAGATTCTGAAGGA |
| CACCACGAAACAAAGGAGGT | |||||
| TRINITY_DN15757_c0_g1_i2 | Protein catecholamines up | 98.4 | 60 | 114 | TTGGGTCTGCGAGTCTTCTT |
| AGCCATTCTCACAGCGAAGT | |||||
| Reference (Xu & Zheng, 2018) | 60S ribosomal protein L6 | 92.1 | 60 | 171 | GGAAGGCACAAGGGAAAGCG |
| CCTGGCTGGGATCTGAACCT | |||||
| Reference (Juárez, 2016) | Elongation factor 1B | 95.3 | 60 | 108 | TGATGTCAAACCATGGGACG |
| AGAGGTGCTAACTTGGACGC |
Note:
E, primer efficiency; Tm, annealing temperature; Size, product size in base pairs.