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Southern blot of total genomic DNA purified from the human isolates digested with either EcoRI (lanes 1 to 5) or BamHI (lanes 6 to 8) hybridized to a PCR-amplified tet(W) probe corresponding to nucleotides 165 to 2113 in the database sequence. Lane 1, B. fibrisolvens 1.230 (positive control); lanes 2 and 6, B. longum F5; lanes 3 and 7, B. longum F8; lanes 4 and 8, F. prausnitzii K10; lane 5, B. fibrisolvens 2221 (Tcs, negative control).
