Table 3.
Performance characteristics of RGN RT-PCR versus reference PCR method.
| Genogroup | TP | FP | TN | FN | Sensitivity (95% CI) | Specificity (95% CI) | % PPV (95% CI) | % NPV (95% CI) | |
|---|---|---|---|---|---|---|---|---|---|
| Children | GI | 18 | 1 | 247 | 0 | 100 (78.1–100.0) | 99.6 (97.4–99.9) | 94.7 (71.9–99.7) | 100 (98.1–100.0) |
| GII | 58 | 0 | 205 | 3 | 95.1 (85.4–98.7) | 100 (97.7–100) | 100 (92.3–100) | 98.6 (95.5–99.6) | |
| Adults | GI | 6 | 5a | 437 | 5c | 54.5 (24.6–81.9) | 98.9 (97.2–99.6) | 54.5 (24.6–81.9) | 98.9 (97.2–99.6) |
| GII | 160 | 7b | 277 | 9d | 94.7 (89.8–97.4) | 97.5 (94.8–98.9) | 95.8 (91.2–98.2) | 96.9 (93.9–98.5) | |
| Children and Adult | GI | 24 | 6 | 684 | 5 | 82.8 (63.5–93.5) | 99.1 (98.0–99.6) | 80 (60.9–91.6) | 99.3 (98.2–99.7) |
| GII | 218 | 7 | 482 | 12 | 94.8 (90.8–97.2) | 98.6 (96.9–99.4) | 96.9 (93.4–98.6) | 97.6 (95.7–98.7) | |
| Overall | GI and GII | 244 | 7 | 453 | 15 | 94.2 (90.4–96.6) | 98.5 (96.7–99.3) | 97.2 (94.1–98.8) | 96.8 (94.6–98.1) |
TP = True positive; FP = False positive; TN = True negative; FN = False negative.
a
Of the 5 GI false positive (including 3 dual positives by the RGN RT-PCR assay), four specimens were classified as GII and one were negative by reference algorithm.
b
Of the 7 GII false positive (including one dual positive by the RGN RT-PCR assay), two specimens were classified as GI and five were negative by reference algorithm.
c
One specimen was misclassified as GII by the RGN RT-PCR assay.
d
One specimen was misclassified as GI by the RGN RT-PCR assay.