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. Author manuscript; available in PMC: 2023 Apr 1.
Published in final edited form as: Circ Res. 2022 Mar 10;130(7):1014–1029. doi: 10.1161/CIRCRESAHA.121.319929

Figure 6: Cxcl8a-Cxcr1 signaling promotes coronary endothelial cell proliferation after cardiac injury in zebrafish.

Figure 6:

A. in situ hybridization for cxcr1 expression on sections of uninjured (A’) and 96 hpci (A”) hearts. Arrowheads point to cxcr1 expression. B. RT-qPCR analysis of cxcr1 and cxcr2 mRNA levels in sorted −0.8flt1:RFP+ cells (cECs) (n=3–4) at 96 hpci normalized to 96 hps. C. Immunostaining of sections of cryoinjured hearts of cxcr1+/+ (C’) and cxcr1−/− (C”) sibling zebrafish at 96 hpci; sections stained for Fli1a (endothelial cells, magenta), PCNA (proliferation marker, green), and DNA (DAPI, blue). Arrowheads point to PCNA+ cECs. D. Percentage of PCNA+ cECs in the border zone of cxcr1+/+ (n=4) and cxcr1−/− (n=4) ventricles at 96 hpci. E. Wholemount images of ventricles of Tg(−0.8flt1:RFP); cxcr1+/+ (E’) and Tg(−0.8flt1:RFP); cxcr1−/− (E”) sibling zebrafish at 7 dpci. F. Percentage of RFP fluorescence intensity in the injured tissue of Tg(−0.8flt1:RFP); cxcr1+/+ (n=3) and Tg(−0.8flt1:RFP); cxcr1−/− (n=3) ventricles at 7 dpci. G. AFOG staining of sections of cxcr1+/+ (G’) and cxcr1−/− (G”) ventricles at 90 dpci. Orange, Muscle; red, Fibrin; blue, Collagen. H. Percentage of scar area relative to ventricular area in cxcr1+/+ (n=4) and cxcr1−/− (n=4) ventricles at 90 dpci. Black (A,G), white (C) and orange (E) dotted lines delineate the injured tissue. Statistical tests: Non-parametric Mann-Whitney test (B), Student’s t-test (D,F,H). Scale Bars: 200 μm (A,E,G), 100 μm (C). Ct values of RT-qPCR data are listed in table S3.