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. 2022 Feb 21;10(4):468–481. doi: 10.1158/2326-6066.CIR-21-0369

Figure 6.

Figure 6. TSPAN7+MECA-79– tumor vessels are associated with lymphocyte clustering and found in TLS. A, IHC staining of different lymphocyte markers was conducted in serial tumor sections to visualize infiltration of T cells, B cells, and Tregs in TLS-free and TLS-rich breast cancer. Tumor specimens of 10 patients were examined for each tumor type (TLS-rich and TLS-free). These tumor specimens were from the same patients assessed in Fig. 1. The consecutive sections of the same tumors were analyzed by immunofluorescence for TSPAN7, MECA-79, and CD31 (lower panels). TSPAN7+MECA-79+ double-positive HEVs (arrows) and TSPAN7+MECA-79– vessels (arrowheads) are both localized in TLS. For the IHC image of the TLS-free tumor, a lower magnification of Fig. 1E is shown. The data shown are for 1 patient representative of each tumor type. Scale bar, 200 µm. B, Immunostaining of MECA-79 (red) and TSPAN7 (brown) in a TLS-rich breast cancer specimen. Leukocytes engrafted in the endothelial wall of TSPAN7highMECA-79+ and TSPAN7highMECA-79– blood vessels are shown. Scale bar, 20 µm. C, Immunostaining of TSPAN7, MECA-79, and sLeX (by F2 antibody) in TLS-rich tumor sections. Arrows show TSPAN7+MECA-79– vessels detected by the F2 antibody. Arrowhead, TSPAN7+MECA-79+ vessels. A representative image of four TLS-rich tumor samples is shown. Scale bar, 100 µm.

TSPAN7+MECA-79– tumor vessels are associated with lymphocyte clustering and found in TLS. A, IHC staining of different lymphocyte markers was conducted in serial tumor sections to visualize infiltration of T cells, B cells, and Tregs in TLS-free and TLS-rich breast cancer. Tumor specimens of 10 patients were examined for each tumor type (TLS-rich and TLS-free). These tumor specimens were from the same patients assessed in Fig. 1. The consecutive sections of the same tumors were analyzed by immunofluorescence for TSPAN7, MECA-79, and CD31 (lower panels). TSPAN7+MECA-79+ double-positive HEVs (arrows) and TSPAN7+MECA-79– vessels (arrowheads) are both localized in TLS. For the IHC image of the TLS-free tumor, a lower magnification of Fig. 1E is shown. The data shown are for 1 patient representative of each tumor type. Scale bar, 200 µm. B, Immunostaining of MECA-79 (red) and TSPAN7 (brown) in a TLS-rich breast cancer specimen. Leukocytes engrafted in the endothelial wall of TSPAN7highMECA-79+ and TSPAN7highMECA-79– blood vessels are shown. Scale bar, 20 µm. C, Immunostaining of TSPAN7, MECA-79, and sLeX (by F2 antibody) in TLS-rich tumor sections. Arrows show TSPAN7+MECA-79– vessels detected by the F2 antibody. Arrowhead, TSPAN7+MECA-79+ vessels. A representative image of four TLS-rich tumor samples is shown. Scale bar, 100 µm.