Fig. 2.

Esomeprazole suppresses migration and autophagy in HTR-8/Svneo cells treated with hypoxia and L-NAME. (a, a′, a″) HTR-8/Svneo cells cultured in transwells were first treated with hypoxia and L-NAME, followed by DMSO or esomeprazole treatment. Transmigrated cells were stained with crystal violet and imaged. (b) Plot showing the quantification of the transmigrated cells. (c) HTR-8/Svneo cells cultured in 6-well plates. After introducing a cell-free gap, the cells were treated with hypoxia and L-NAME, followed by DMSO or esomeprazole treatment. Cell migrations were recorded at 24 h and 48 h. (d) Plot showing the quantification of the healing rate. (e) LC3B, P53, and PI3KC3 proteins levels were detected via Western blot. (f–f‴) Plot depicting the quantification of LC3B, P53, and PI3KC3 expression levels. *P < 0.05, **P < 0.01