Fig. 8.

Esomeprazole-suppressed autophagy induced by L-NAME via AMPKα-mTOR in PE mice. (a) LC3B proteins levels in placentas of control, L-NAME-treated, or L-NAME + esomeprazole-treated mice were detected by Western blot. (a′) Plot depicting the quantification of LC3B protein levels. (b–d″) LC3B in placentas of control, L-NAME-treated, or L-NAME + esomeprazole-treated mice were evaluated by immunofluorescence staining. (e) The protein levels of phospho-AMPKα, AMPKα, PPARγ, SirT1, mTOR, and phospho-mTOR in placentas of control, L-NAME-treated, or L-NAME + esomeprazole-treated mice were detected by Western blot. (f–f⁗) Quantification of the LC3B, HIF1α, pAMPKα/AMPKα, PPARγ, and phospho-mTOR/mTOR in placentas of control, L-NAME-treated, or L-NAME + esomeprazole-treated mice, N = 3 mice per group, *P < 0.05, **P < 0.01