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. Author manuscript; available in PMC: 2023 Apr 1.
Published in final edited form as: J Immunol. 2022 Mar 18;208(7):1595–1615. doi: 10.4049/jimmunol.2100439

FIGURE 1. Immunophenotyping of cellular sources of IL-22 in human decidual tissues.

FIGURE 1.

(A) Spatial localization of the human decidua basalis and parietalis. (B) Gating strategy used to identify neutrophils (CD14−CD15+), macrophages (CD15−CD14+), T cells (CD14−CD15−CD19−CD3+), B cells (CD14−CD15−CD3−CD19+); NK cells (CD14−CD15−CD3−CD19−CD56+), and innate lymphoid cells (ILCs; CD14−CD15−CD3−CD19−CD11b−CD56−(Lin−)CD127+) in the human decidua parietalis and basalis. (C) Representative t-SNE plot showing the relative distribution of immune cell populations in the human decidual tissues. (D) Proportions of IL-22+ T cells in the decidua parietalis of women with term labor (TIL, n = 29) or preterm labor (PTL, n = 26) and decidua basalis of women with TIL (n = 30) or PTL (n = 26). (E) Proportions of IL-22+RORγt+ T cells in the decidua parietalis of women with TIL (n = 29) or PTL (n = 26) and decidua basalis of women with TIL (n = 30) or PTL (n = 26). The p-values were determined using Mann-Whitney U-tests. Data are shown as scatter plots with medians, interquartile ranges, and min/max ranges. Demographic and clinical characteristics of the study population are shown in Table I. (F) Representative t-SNE plots showing the relative distribution of RORγt, CD69, CD127, IL-17A, IFNγ, IL-5, and IL-13 expression by IL-22+ T cells in the human decidual tissues. (G) Overview of the single-cell RNA-sequencing (scRNA-seq) of the human decidual tissues showing the major cell types identified in a Uniform Manifold Approximation Plot (UMAP) as reported in (68). Gene expression (scaled to transcripts per million) of IL22, Il22RA1, IL10RB, IL22RA2, and RORC by immune cells in the (H) basal plate and (I) chorioamniotic membranes of women who delivered at term without labor (TNL, n = 3), TIL (n = 3), or PTL (n = 3). Dot size corresponds to the proportion of cells expressing each transcript within a given cell type.