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. Author manuscript; available in PMC: 2023 Apr 1.
Published in final edited form as: J Immunol. 2022 Mar 18;208(7):1595–1615. doi: 10.4049/jimmunol.2100439

FIGURE 6. IL-22 is expressed by neutrophils and monocytes/macrophages in the human and murine amniotic cavity upon microbial infection.

FIGURE 6.

(A) IL-22 participates in the local host immune response to microbial invasion of the amniotic cavity. (B) Concentrations of IL-22 in the amniotic fluid of mice intra-amniotically injected with Ureaplasma parvum (n = 12) or SP4 broth (n = 6). P-value was determined using a Mann-Whitney U-test. (C) Linear regressions showing the correlations between amniotic fluid concentrations of IL-22 and those of IL-6, IL-21, and IL-17A in mice intra-amniotically injected with Ureaplasma parvum (n = 8). (D) Representative immunofluorescence imaging of amniotic fluid neutrophils and monocytes/macrophages from mice intra-amniotically injected with Ureaplasma parvum showing the single and merged expression of DAPI (nuclei, blue), Ly6G (neutrophils, green), F4/80 (monocytes/macrophages, red), and IL-22 (yellow). Representative immunofluorescence imaging of human amniotic fluid (E) neutrophils and (F) monocytes/macrophages obtained from women with intra-amniotic infection showing the single and merged expression of DAPI (blue), CD15 (neutrophils, violet), CD14 (monocytes/macrophages, red), and IL-22 (green). Green boxes correspond to cells of interest. All images taken at 40X magnification. Scale bars indicate 20μm.