Fig. 4.

Intracellular uptake and distribution of FAM-siRNA and DiD in MCF-7 cells after an incubation with double-labeled liposomes for (a) 3 h and (b) 6 h. The FAM-siRNA and DiD concentrations were 400 nM and 680 ng mL⁻¹, respectively. Scale bar, 7.5 µm. Cell nuclei and early endosomes were counterstained with Hoechst 33,258 (blue) and CellLight Early Endosomes-RFP BacMam 2.0 (red), respectively. FAM-siRNA fluorescence (green) and DiD fluorescence (red) were recorded