Figure 4.

Estrogen promotes GCN5L1 translation. A: expression of GCN5L1 mRNA levels determined by qPCR after treatment with 17β-estradiol (E₂) or G-1. n = 3. B: GCN5L1 levels are significantly elevated by blocking 26S degradation, suggesting that G protein-coupled estrogen receptor (GPER) agonism does not increase GCN5L1 via impaired proteolysis. n = 4, *P < 0.05, ***P < 0.001 vs. control (two-way ANOVA with post hoc Tukey’s multiple comparison’s test). C: GCN5L1 induction is blocked by cycloheximide (CHX), a translation inhibitor. n = 4, *P < 0.05, ***P < 0.001 vs. control (two-way ANOVA with post hoc Tukey’s multiple comparison’s test). D: proposed mechanism of action.