Figure 3.

With 3 days of Na⁺, K⁺, and Cl⁻ restriction, β- and γ-epithelial Na⁺ channel (ENaC) subunit abundance is lower in male pendrin-null [knockout (KO)] mice than in wild-type (WT) mice. Pendrin-null and WT mice were given 4 days of the NaCl-replete, K⁺-restricted diet (treatment 2), and then 3 days of the Na⁺, K⁺, and Cl⁻-restricted diet (treatment 1). As shown, although α-ENaC subunit abundance was similar in both groups (A–C), the abundance of both β-ENaC (D and E) and the 70- and 85-kDa fragments of γ-ENaC (F–H) were lower in kidneys from pendrin-null than from WT mice. Open circles represent individual data points for WT mice (left) and pendrin-null (right) mice. *P < 0.05 (unpaired Student’s t test).