Figure 5.

Following dietary Na⁺, K⁺, and Cl⁻ restriction, apparent intravascular volume depletion is greater in male than in female pendrin-null [knockout (KO)] mice. Male and female wild-type (WT) and pendrin-null littermates were given a NaCl-replete, K⁺-deficient diet (treatment 2) for 4 days, and then 7 or 8 days of the Na⁺, K⁺, and Cl⁻-deficient diet (treatment 1). A: weight change over the 12-day treatment period in mice from these four groups. Blood pressure was compared in the same mice after the NaCl-replete, K⁺-deficient diet, and then the Na⁺, K⁺, and Cl⁻-deficient diet using a paired Student’s t test (B). Blood urea nitrogen (C) and arterial blood gases (D) were measured at the end of the 12-day treatment period in mice from each group. Multiple comparisons were made with one-way ANOVA and a Holm–Sidak posttest (A and D). Values in C were compared using Kruskal–Wallis one-way analysis of variance on ranks. *P < 0.05.