Table 1.
Effect of pendrin gene ablation on serum aldosterone and electrolytes in mice fed a NaCl-replete, K+-deficient diet for 4 days, and then either 3 or 8 days of a Na+, K+, and Cl−-deficient diet
| Strain | n | Na+, meq | K+, meq | Cl−, meq | Aldosterone Treatment |
|
|---|---|---|---|---|---|---|
| n | nM | |||||
| Four days of a NaCl-replete, K+-deficient diet (treatment 2) and then 3 days of a Na+, K+, and Cl−-deficient diet (treatment 1) | ||||||
| Males | ||||||
| Wild type | 9 | 141 ± 1 | 3.5 ± 0.1 | 110 ± 1 | 5 | 1.72 ± 0.19 |
| Pendrin KO | 8 | 136 ± 0.3* | 3.1 ± 0.1* | 100 ± 1* | 5 | 2.98 ± 0.38* |
| Females | ||||||
| Wild type | 7 | 142 ± 1 | 3.9 ± 0.1 | 109 ± 1 | NM | |
| Pendrin KO | 7 | 137 ± 1* | 3.2 ± 0.2* | 98 ± 2 | NM | |
| Four days of a NaCl-replete, K+-deficient diet (treatment 2) and then 8 days of a Na+, K+, and Cl−-deficient diet (treatment 1) | ||||||
| Males | ||||||
| Wild type | 14 | 145 ± 1 | 4.1 ± 0.1 | 113 ± 1 | 5 | 1.28 ± 0.13 |
| Pendrin KO | 13 | 138 ± 1* | 3.0 ± 0.1* | 97 ± 1* | 5 | 1.57 ± 0.21 |
| Females | ||||||
| Wild type | 11 | 142 ± 1 | 4.0 ± 0.1 | 112 ± 1 | 5 | 1.53 ± 0.06 |
| Pendrin KO | 12 | 137 ± 1* | 3.0 ± 0.1* | 99 ± 1* | 5 | 2.65 ± 0.22* |
Values are means ± SE; n, number of mice studied. Mice were on a C57Bl/6 background. An unpaired Student’s t test was used to compare wild-type and knockout (KO) mice of the same sex. *P < 0.05. NM, not measured.